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While post-exercise heart rate (HR) variability (HRV) has been shown to increase in response to training leading to improvements in performance, the effect of training leading to decrements in performance (i.e., overreaching) on this parameter has been largely ignored. This study evaluated the effect of heavy training leading to performance decrements on sub-maximal post-exercise HRV. Running performance [5 km treadmill time-trial (5TTT)], post-exercise HRV [root-mean-square difference of successive normal R-R intervals (RMSSD)] and mea