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Sequencing of different tissues should be considered when there is conflict between the UBA1 variant status and the clinical findings. The present case exemplifies the diagnostic challenge that was imposed by the high VAF detected by ES that failed to correctly demonstrate that the variant was in a mosaic state. Sequencing of different tissues should be considered when there is conflict between the UBA1 variant status and the clinical findings. To investigate the effect and clinical significance of different thoracic surgical approaches f