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ages according to the Sarnat and Sarnat staging system stage I mild (irritable, normal, or hypertonia and poor feeding); stage II moderate (lethargy, hypotonia, and frequent seizure); stage III severe (coma, flaccid, absent reflexes, and frequent seizure). There is a positive association between LDH levels and the severity of HIE. The influences of methanol proportion, flow rate, column temperature, and injection volume in the mobile phase on the chromatographic resolution of chromatographic peak of euphorbia factor L1 were experimentally studied v