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In addition, HPLC-ELSD has been established as the internationally acceptable measure of rhamnolipids for commercial purposes. The preparation of well-accepted rhamnolipids standards and modifications of analysis operations are essential to further enhance the accuracy and improve the simplicity of rhamnolipid analysis.Key points• Current status of RD works on determination of rhamnolipids is listed• Advantages and disadvantages of various types analysis are summarized• Limitations of current rhamnolipid quantification are discusse