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Further observation with laser confocal microscopy and transmission electron microscopy was conducted. It was found that compared with the wild type, the number of vesicles in BcSec3 deleted cells reduced and localization and distribution of vesicles changed. In mutant cell, vesicles relatively concentrated in the cytoplasm, while in wild-type cell mainly concentrated inside the cell membrane. These evidences indicate that the exocyst subunit BcSec3 plays an important role in the growth, development and pathogenicity of B. cinerea. Infec
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