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Knowledge received from such researches can be placed on epidemiological danger assessments for the various transmission roads. Such tests can be employed to aid predict the most effective biosecurity and control techniques. © 2020 Blackwell Verlag GmbH.We evaluated 345 wild pets from southern and south-eastern Brazil to know their particular part in vaccinia virus (VACV) transmission cycle. VACV DNA was recognized in rats, marsupials, chiroptera and cingulate, expanding the knowledge of VACV n