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The mushroom P. citrinopileatus has strong ability to uptake selenium from the surroundings, which modifies many biological pathways. This paper provides a theoretical reference for the development of Se-enriched fungal foods.Phallus atrovolvatus is a wild edible mushroom found in Thailand. Three strains of Ph. atrovolvatus (DOAP-1, DOAP-2, and DOAP-3) were collected from forests in Central Thailand. Some requirements for mycelial growth were obtained in different media. Potato dextrose agar was determined as the best medium to support mycelial grow