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The male reproductive systems of A. mixtum and R. sanguineus s.l. were fixed in 2.5% glutaraldehyde for 48 h and dehydrated in increasing dilutions of ethanol. The samples were then embedded and mounted in historesin to obtain sections of 3 μm that were stained with hematoxylin-eosin (HE), photographed, and visualized through optical microscopy. The results show that the morphology of mature germ cells displays excellent diagnostic traits that can be used for tick taxonomy.Elevated atmospheric CO2 concentration increases the perfor