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HPTLC method was also validated for the quantification of RA in the extracts of investigated herbs. RA contents decreased in such a sequence (24.83 ± 0.78mg/g) (20.32 ± 0.64mg/g) (19.59 ± 0.61mg/g) (18.77 ± 0.52mg/g) (12.61 ± 0.43mg/g). Obtained data can facilitate the differentiation of investigated species using the chromatographic fingerprints of their phenolic compounds. Developed and validated HPTLC method provides an approach to estimate RA content as a common marker of investigated herbs. Obtained data can facilitate the differe