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An electron-assisted strategy was developed to prepare gold nanoparticles (AuNPs) at room temperature. Glow discharge plasma as electron source was successfully used to control the valence state, size, and shape of AuNPs. Stable Au(I) was obtained in 3 min by plasma, and Au(I) was reduced to zero valence with the increase in treatment time. An increase in the amount of Au did not induce an increase in particle size. A narrow size distribution was also achieved. The narrowest size distribution was observed at 9 min at 600 V. AuNPs grew sl