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392). Change in FMD was significantly different between the groups (ΔFMD vs. ΔFMD , p=0.032). There was a statistically significant correlation between automatically analysed results and those obtained by an experienced technician (FMD r=0.822, p0.001; FMD r=0.645, p=0.007). The influence of NO on FMD is approximately 40% if assessed using the UNEX EF. Prior to use FMD as a marker of endothelial dysfunction, we should explore different methods including various duration of forearm ischaemia to increase NO dependency of FMD. The influ