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The four LbESTs contain a catalytic triad (Ser-His-Glu) linked to an oxyanion gap and acyl pocket involved with substrate stabilization during its hydrolysis. Synergism observed with the esterase-inhibitor DEF shows the participation of esterases in malathion detoxification. LbESTs were expressed through the entire of developmental phases, but prevalent abundance in the 1st nymphal instar and person phase. The mRNA degree of three LbEST genes (except for LbEST4) had been c