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To evaluate our hypothesis, we utilized an in vitro study model that mimics COPD exacerbations and performed considerable scientific studies to know the role of CpG promoter methylation of NF-κB and STAT3-mediated path genes. Both NF-κB and STAT3 transcription factors play important roleOPD exacerbation study design. More, we observed an important role of TET1/2 in regulating the DNA methylation of NF-κB, STAT3, IKK, and NIK genes and cytokine/chemok