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This quantification approach was successfully applied and validated with field sample results and is consistent with the well-established environmental stability of these petroleum biomarkers and modern hydrocarbon fingerprinting methodology.Ten global harbours were assessed for sediment quality by quantifying the magnitude of anthropogenic change and ecological risk. Anthropogenic change (enrichment) was high for Derwent River and Sydney estuary, moderate for Santander Harbour, Rio de Janeiro and Dublin Port, slight for Hong Kong, minimal