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Bioaccumulation of rock and metalloid in the tubifex tissue and alter of native microbial neighborhood under treatment of different biochars were examined. Biochars created at 700 °C exhibited greater effect on lowering the concentrations of As and Cd in aqueous period, and TCLP extractable and bioavailable metal(loid) in solid stage of sediment. The focus of As and Cd in water period diminished by 26%-89% and 22%-71% under the remedy for straw biochar, and reduced by