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5 and 100% and specificities of 97.3 and 93.2%, respectively. Post-TSH T4 levels of 1.7μg/dL had a negative predictive value of 100%. Post-TSH T4 levels of 1.3μg/dL showed a positive predictive value of 94.9%. Area under the ROC curve for post-TSH T4 was 0.99. A TSH stimulation test performed with a recombinant human TSH dose of 75 μg/dog is highly reliable to discriminate between hypothyroid and euthyroid dogs, even in cases of concurrent non-thyroidal illness or administration of medications. A post-stimulation T4 concentration