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Methanogenesis ended up being seen in duplicate microcosms that were unamended, amended with sulfate, or which were initially oxic, but methanogenesis was not observed in nitrate-amended controls. The best rate of methane manufacturing had been 0.15 μmol CH4 g-1 oil d-1 , instructions of magnitude less than other reports of methanogenesis from lighter crude oils. Methanogenic Archaea and many possib