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We demonstrate the potential of photothermal deflection spectroscopy (PDS) to study the self-assembly of dye monolayers in situ. Beyond the determination of adsorption kinetics at specific wavelengths, PDS gains its strength from yielding UV-vis absorptance spectra of SAMs in situ, unaffected by scattering, from which supramolecular interactions can be deduced.A rapid and sensitive approach for enriching and extracting triazines from brown sugar samples was developed by combining magnetic dispersive solid-phase extraction and HPLC/UV. In