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Due to the biochemical and pharmacological activities, the convenient and effective detection of quercetin (Qc) is very important for biochemistry, pharmaceutical chemistry and clinical medicine. A kind of non-conjugated polymer dots (NCPDs) was used as a versatile and sensitive dual-mode optical output for Qc detection, which was synthesized by hyperbranched poly(ethylenimine) (PEI) and l-threonine via environmentallyfriendly way. The dual-mode method proposed in this work had high sensitivity and definiteselectivity for Qc detection. Additionally, it was