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Peripheral blood mononuclear cells (PBMC) were split into 3 wells for each topic when you look at the existence of 1,25 vitD3 (50 nM, for 16 hours), PHA (positive control) (10µM) and with no treatment (as a baseline or negative control). The percentage of regulating T cells and Th17 cells had been assessed by movement cytometry at standard after which after cell tradition experiments. RESULTS Our research suggested that the percentage of Tregs when you look at the cus