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Our evaluation disclosed that treatment with 1000 ppm CO2 would not affect stomatal figures on abaxial edges of cotyledons but increased cotyledon area, causing reduced stomatal thickness, seven days after germination. Interestingly, this treatment also perturbed the uniform distribution of stomata via excess satellite stomata and stomatal precursor cells. We used overexpression lines of the DNA replication licensing aspect gene CDC6, a reported good regulator of satellite stomata manufacturing. CDC6 overexpression decreased the rate of