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We created a hetero-cellular spheroid system composed of major individual hepatocytes (PHH) co-cultured with crude fractions of primary man liver non-parenchymal cells (NPC) from a few coordinated or non-matched donors, to identify phenotypes with utility in examining NASH pathogenesis and drug evaluating. Co-culture spheroids exhibited steady expression of hepatocyte markers (albumin, CYP3A4) with the integration of stellate (vimentin, PDGFRβ), endothelial (vWF, PECAM1), a