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, transglutaminase (TGase)), and thus achieves building of centimeter-sized structure with high cell viability and technical security in vitro within 30 min. Further in vivo implantation associated with 3D-MAPS-fabricated ear-like muscle construct in rabbit designs assisted by flap prefabrication strategy outcomes in increased structural vascular support and strengthened useful success. Thus, the TGase-enhanced 3D-MAPS shows its potential and