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Importantly, the GFP + cells isolated from the mdx mice (paired with GFP mice) underwent myogenic differentiation in vitro and expressed markers of mesenchymal stem cells and macrophages, which may potentially be involved in the improvement of dystrophic muscle pathology. These observations suggest that changing the dystrophic microenvironment can be a new approach to treat DMD.Although protected areas (PAs) are declared to provide sanctuaries for biodiversity, they are increasingly threatened by the synergistic effects of anthropic fac