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HDF is achieved making use of buffer liquid to sheath the test fluid, requiring four liquid harbors to use by pressure driven circulation. A low-pressure chamber, or pit, formed by etching into a substrate, makes it possible for volumetric flow ratio-induced concentrating at a reduced movement velocity. The single-layer design simplifies surface micromachining and gets better product yield by 1.56 times over previous work. The concentrating design was integrated with optical waveguides and uti
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