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Most readily useful outcomes were acquired by utilizing a buffer consisting of 10 mmol/L Cu(II), 13 mmol/L L-histidine, 8 mmol/L β-CD, 5 mmol/L phosphate adjusted to pH 5.0 and 15 kV applied voltage. All enantiomers were totally resolved within 20 min with high resolutions of 3.6~6.1. The analysis technique was confirmed through the dedication of D,L-tryptophan with regards to linearity, accuracy and reliability. In addition to robustness of the technique e