https://www.selleckchem.com/ph....armacological_epigen
Most of these studies concluded that OPU-DN time did not influence ICSI outcomes, but some suggested that oocytes should be incubated for a short time before DN to improve oocyte maturity and enhance ICSI outcomes. In addition to reports on positive or negligible effects, adverse effects were reported in 12 studies on DN-ICSI timing. Neither OPU-DN nor DN-ICSI time could improve live birth rate. Oocytes should be pre-incubated for a short duration (preferably 4 h) before DN according to the ART laboratory schedule. More
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