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The major chemical marker of black pepper (Piper nigrum L) is piperine and its estimation is extremely important for quality assessment of black pepper. The methods for piperine quantification, to date, are laboratory based and use high end instruments like chromatographs, which require tedious sample processing and cause sample destruction. In this article, we present a simple, rapid and green analytical method based on Raman spectroscopy for the quantitative assessment of piperine. To assess the potential of the technique, we report the complete