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Next-generation sequencing showed a novel compound heterozygous CFTR mutation (c.400 A G p.Arg134Gly and c.3484 C T p.Arg1162 ∗ ) which led to CF when you look at the household. Conclusions. As this mutation is consistent with the noticed medical manifestations of CF with no various other mutations had been recognized after checking the gene sequence, we claim that their particular CF phenotypes tend to be due to the substance heterozygous mutation, c.400 A G p.Arg134Gly a