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Mice were split into four groups CE0, GDME0, CE18, and GDME18. C and GDM represent the control (C) and GDM groups, while E0 and E18 represent very early or belated trimesters of embryo day 0 or 18, respectively. GDM mouse models were developed by injecting streptozocin on embryo time 0. The gut microbiota had been characterized using the Illumina MiSeq system concentrating on the V3-4 area regarding the 16S rRNA. Operational taxonomic device evaluation disclosed a difference between