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High-throughput sequencing showed that IMO and INU increased the faecal microflora α- and β-diversity (P less then 0.05). Methanobrevibacter was more abundant (P less then 0.05), whereas the richness of Turicibacter was lower in the INU and IMO groups than in the control group (P less then 0.05). In addition, IMO significantly increased litter size (P less then 0.05). Overall, our findings indicate that INU and IMO can relieve constipation, optimise intestinal flora, and promote reproductive performance in pregnant sows.In an effort to ex