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Statistically significant differences were observed in relation to total protein. (4) Conclusions Saliva of e-cigarette users presents changes in physicochemical composition in comparison to traditional cigarette smokers and non-smokers, however, statistically significant differences were observed only in calcium concentration. Further longitudinal studies on a larger study group should be conducted to assess the effect of observed changes in oral health.During Action Observation (AO), patients observe human movements that they then t