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© 2020 Wiley Periodicals LLC. Basic Protocol 1 Construction of CRISPR/Cas9 targeting vectors Support Protocol 1 Choosing protospacers in the target gene Basic Protocol 2 Agrobacterium-mediated transformation of Blastomyces Support Protocol 2 Preparation of electrocompetent Agrobacterium Support Protocol 3 Preparation and recovery of Blastomyces frozen stocks.Within the short timeframe of the COVID-19 pandemic, there has been increasing interest in its potential impact on psychological stress and sleep. Using standardi