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The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. Five routines were performed, each consisting of eight epididymis and the structures were sliced onto a glass plate containing a commercial diluting medium for Botubov. The samples were divided into four groups, with 80 x 10 spermatozoa per mL. Group 1 s