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How sediment bacterial community structure and diversity responds to different gradients of nitrogen (N) in swine wastewater is poorly understood. Here, the effects of different total nitrogen (TN) concentrations in swine wastewater on the microbial diversity and community composition in surface flow constructed wetlands (SFCWs) were investigated. The five concentration gradients included 2, 250, 300, 350, and 400 mg L-1. Under high N concentrations (300 mg L-1), the Ace and Chao1 indexes increased, however, the Shannon index decline