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). Peromyscus ear tissue and blood samples were tested for Bo. burgdorferi sensu stricto (s.s), A. phagocytophilum, Ba. microti, and Borrelia miyamotoi. We found 13.6% (15/11 of questing I. scapularis nymphs to be Bo. burgdorferi s.l. positive and 1.8% (2/11 were A. phagocytophilum positive among all sites. Borrelia burgdorferi s.s. was found in 71.1% (54/76) of I. scapularis nymphs removed from mice and 58.8% (194/33 of captured mice. Results from study on tick abundance and pathogen infection status in questing ticks, rodent res