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In this research, residual αGal within the DCM was first dependant on an enzyme-linked immunosorbent assay method with skilled reliability and specificity. Then DCM was implanted subcutaneously in to the α1,3-galactosyltransferase gene-knockout (GTKO) mice, accompanied by the implantation within the wild-type C57BL/6 mice as an evaluation. The sum total serum antibody levels, anti-Gal antibody levels, inflammatory cytokines and ratios of splenic lymphocyte subtypes had been detect
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