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The purpose of this study was to detect the mechanical anisotropy of the cornea using Brillouin microscopy along different perturbation directions. Brillouin frequency shift of both whole globes ( = 1 and cornea punches ( = 1 were measured at different angles to the incident laser, thereby probing corneal longitudinal modulus of elasticity along different directions. Frequency shift of virgin ( = 26) versus cross-linked corneas ( = 15) over a large range of hydration conditions were compared in order to differentiate the contributi