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BACKGROUND Circular RNAs (circRNAs) are a special type of non-coding RNA. To elucidate the relationship between hemodynamics and the function of circRNAs in endothelial cells (ECs), a modified T chamber system was designed and produced for the present experiment. This T chamber system can be used to simulate the hemodynamic environment at the bifurcation of the arteries. METHODS Normal ECs cultured on glass slides were placed in the T chamber, the cell layer was impacted at a flow rate of 500 mL/min, and high-throughput microarrays wer