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All 12 isolates harboured a plasmid carrying the Tn1-bla element, although they were slightly different in size (78 883 bp, 78 898 bp and 78 913 bp) owing to differences in the number of 15-bp repetitive sequences. A 42 542-bp broad-host-range plasmid carrying the Tn1-bla element was also found in one of the isolates. We characterised a plasmid-encoded novel Tn1-bla element and transposon-dependent mechanisms underlying the propagation of antibiotic resistance, together with repeated new polymorphic 15-bp units in the promoter of bla . We