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Temperature influences all biochemical and biophysiological processes within an organism. By extension, it also affects those ecological interactions that are mediated by gland-produced chemical compounds, such as reservoir-based chemical defense. Herein, we investigate how environmental temperature affects the regeneration of defensive secretions and influences the efficacy of chemical defense in a model predator-prey system the oribatid mite Archegozetes longisetosus and the predaceous rove beetle Stenus juno. Through a combination of c