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Simultaneously, the control experiment was create with the exact same protectant and conservation time. Bacteria powders were maintained using cleaner lyophilization and preserved at 4 °C for 60 times. During the 54 times of restoration, the reactors that were inoculated with micro-organisms dust preserved by different ways revealed factor. The results show that the anammox micro-organisms dust with 3 wt% DMSO as the cryoprotectant and with no substrate
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