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An insect's ability to tolerate winter conditions is a critical determinant of its success. This is true for both native and invasive species, and especially so in harsh polar environments. The midge Eretmoptera murphyi (Diptera, Chironomidae) is invasive to maritime Antarctic Signy Island, and the ability of fourth instar larvae to tolerate freezing is hypothesized to allow the species to extend its range further south. However, no detailed assessment of stress tolerance in any other life stage has yet been conducted. Here, we report
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