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https://www.selleckchem.com/pr....oducts/bgj398-nvp-bg
To establish cultures of human lacrimal gland from patient-derived, biopsy-sized, tissue specimens. Tissue was obtained after surgical removal from patients without dry eye disease undergoing routine procedures. Samples were subjected to mechanical and enzymatic digestion and resulting cell suspensions were plated onto collagen-coated glass coverslips and grown for up to 21 days. Cultures were analysed by immunocytochemistry and light microscopy, and resultant cellular distributions were compared to those in sections of fixed