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A well balanced pig tonsil mobile line is essential for JEV persist infection research. In this research, we established a continuous epithelial mobile range, called PT cell, through the pig tonsil. This mobile is vunerable to JEV. We determined the growth characteristics, molecular properties, microstructure profiles of PT cellular. JEV is simple to enter PT cellular which could partially explain the reason of persist illness. We further determined that LMAN2L, a mannose lectin proteins, is t