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OBJECTIVE This study aimed to elucidate the role of decorin, a small leucine-rich proteoglycan, in the degradation of cartilage matrix during the progression of post-traumatic osteoarthritis (PTOA). METHODS The destabilization of the medial meniscus (DMM) surgery was applied to 3-month-old decorin-null and inducible decorin knockout mice to induce PTOA. The resulted OA phenotype was evaluated by assessing joint morphology and sulfated glycosaminoglycan (sGAG) staining via histology (n = 6/group), surface collagen fibril nanostructure vi