https://www.selleckchem.com/pr....oducts/jnj-64619178.
API ZYM kit was used to compare the intracellular enzyme activity of S. aureus in the exponential phase with that in the VBNC state. The results showed that the enzyme activity decreased significantly in VBNC cells, and that the VBNC cells were more resistant to simulated gastrointestinal fluid through flow cytometry analysis. Quantitative reverse-transcription polymerase chain reaction results suggested that the ability of adhesion and biofilm formation of VBNC cells might be decreased due to the down-regulation of related genes.
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