https://www.selleckchem.com/pr....oducts/Nanchangmycin
All the analytes were separated on a C18 reversed phase column, eluted with a mobile phase consisting of acetonitrile-formic acid (0.01%) (25 75, v/v), and detected by ESI in the selected ion mode with multiple reaction monitoring (MRM). The target fragment ions were m/z 525.3 ⟶ 449.5 for PF, 336.2 ⟶ 320.2 for BBR, and 180.1 ⟶ 163.1 for IS. The linear ranges of PF and BBR were 5-500 ng/mL and 0.1-20 ng/mL with good linearity (r2 0.99). No obvious matrix effect was observed, and acceptable accuracy, precision, recovery, and s
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