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The selectivity and sensitivity of the method were also determined. The AOAC 996.06 method is fit for purpose for the analysis of DHA in dry dog food kibble. The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternative manufacturing methods, i.e. pelleted and extruded with no significant matrix effects being observed. The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternativ