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The magnitude of the differences was less than 0.5μm. The results of the ANOVA demonstrated that the individual factors fluoride concentration and number of strokes were significant (P0.05), however, the method of analysis was not (p=0.74). ICC's between the two methods of analysis were excellent (0.996, p0.001) with no proportional bias. This study reports that step height on dentine from a digital microscopy and non-contact profilometry were not significantly different. The digital microscope, although slower, allows visual inspec